A Note on Urea Estimation by Diacetyl Monoxime

A note on urea estimation by diacetyl monoxime.

Rosenthal (1955) has described in great detail a method using diacetyl monoxime which is generally satisfactory for the routine estimation of urea in blood and urine except that complete colour development requires prolonged heating in boiling water. This disadvantage can be overcome by the use of a pressure cooker. The tubes of reaction mixture are immersed in a beaker filled with hot water to...

The automated thiosemicarbazide-diacetyl monoxime method for plasma urea.

IN RECENT YEARS the automated diacetyl monoxime (DAM) reaction as adapted to the AutoAnalyzer has become the method of choice in many clinical laboratories for the determination of urea in biologic fluids. The method is based on the condensation of urea with diacetyl, the latter usually being liberated from the monoxime in the presence of one of various acid reagents (1, 2). The reaction, howev...

A study of the reaction of urea with diacetyl monoxime and diacetyl.

Improvements in the determination of urea using diacetyl monoxime; methods with and without deproteinisation.

A rapid and reproducible method is described for measurement of urea in biological materials (after deproteinisation) and in serum (without deproteinisation). Urea is colorimetrically determined with diacetyl monoxime and thiosemicarbazide in the presence of sulphuric acid, phosphoric acid and ferric chloride. The sensitivity of the colorimetric reaction and stability of the colour are enhanced...

Determination of Urea in Blood and Urine with Diacetyl Monoxime-glucuronolactone Reagent.

N UMEROUS STUDIES have been reported in the literature on the photometric determination of urea in biological fluids with diacetyl monoxime as the color-developing agent. The reaction is photosensitive, does not follow Beer’s law, and requires special precautions for reproducible results. In many proposed procedures, oxidants were added in the reaction mixture to destroy hydroxylamine which mig...